Fig. 1.
TbAU1K is essential for infection in mice. Mice were inoculated ip with 3×106 TbAUK1 RNAi cells. One group containing three mice received 1 mg/ml doxycycline in the water at day 0 (+ Dox). The control group comprised of two mice received water without doxycycline (- Dox).
(A) Parasiemia was monitored in peripheral blood at the times indicated. Each line plots the infection in a single mouse. The crosses indicate that the mice died within 24 hours of the last recorded parasitemia. The detection limit of the assay is 2×105 cells/ml.
(B) After 3 days of growth in a separate doxycycline treated mouse, trypanosomes were examined in the blood by DIC, or were labeled with antibodies against PFR (red) and counterstained with DAPI (blue). The cells at upper right were viewed at 60× while cells in the lower panels were viewed at 100×. The lower right panel shows two cells, each of which has multiple flagella and multiple kinetoplasts. The bars are size markers of 10 μm. Substrate specificities of TbAUK1 and TbAUK3.