TABLE 1. Primer pairs used for RNAi and RT-PCR analyses.

PCR primers are listed 5′ to 3′ with restriction sites in bold, the AU1 tag underlined and mutations italicized in lower case. When gene fragments were cloned, the sequence positions of the primers are listed.

Primer Pairs	Sequence
pZJM.TbAUK1
Fwd: XhoI 105-127	ATCGCTCGAGTGGCGGCGGCAATTACGGGGATG
Rev: HindIII 615-637	ATCGAAGCTTTGCCCAGGCACCAAAGGTCAGCAC
pHD496.AUK1-TbAUK1	ATCGAAGCTTATGGACACGTACCGCTACATTAGGTCAAC
Fwd: HindIII	TGAGGTCGGGCGTGTT
Rev: BamHI	ATCGGGATCCTCAATTCTCTTTCCCTGCAGTTGGCTCTGC
K58R TbAUK1
Fwd: BssHII 159-211	TTTGTTTGCGCGCTGcgcAGGTTGTCCATTTAAAAAACTTG CGGATTTTGACAT
pLEW100.TbAUK1-AU1
Fwd: HindIII	ATCGAAGCTTATGAGGTCAACTGAGGTCGGGCGTG
Rev: BamHI	ATCGGGATCCTCAAATGTAGCGGTACGTGTCATTCTCTTT CCCTGCAGTTGGCT
TbAUK1-RT
Fwd: 654-677	ATCGTCTCGTTGGTAAAACACCGTTTGT
Rev: 834-857	ATCGGGGAGATAATAATACTTGAGAAGG
Carbonic Anhydrase-RT
Fwd: 84-107	ATCGCACGGCAGGAATGGGGCGGGCGGA
Rev: 284-307	ATCGCGCCGTTGTCGATTTTTAGGGAAG
Dynein Heavy Chain-RT
Fwd: 313-336	ATCGCGCCGCCGCCGACTATACGAATCG
Rev: 494-517	ATCGCGGCGGGAAGGTAGCGACCAGCGG
Alpha Tubulin-RT
Fwd: 11-34	ATCGCTATCTGCATCCACATTGGTCAGG
Rev: 516-539	ATCGAGCCGTCGACACCTGCGGTGATGG