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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Jan 13;1791(3):198–205. doi: 10.1016/j.bbalip.2008.12.018

Figure 1. ABHD5-shRNA reduced protein levels of ABHD5 by 90%.

Figure 1

McA RH7777 cells were transduced with either ABHD5-shRNA or control-shRNA, or were not transduced. Cells were harvested after 48 hours of growth in serum-containing culture medium without supplemental fatty acids. The expression of ABHD5 and β-actin were analyzed by immunoblotting (B). Expression of ABHD5 was quantified by scanning densitometry and expressed relative to controls (A). The results represent the means and standard deviations from a representative experiment with triplicate samples. The experiment was repeated five times with similar results. Statistical analysis was performed using one-way ANOVA and Bonferroni’s post-hoc test; *** indicates significant differences at p < 0.001.