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Defining the SLM-2 response element as a bipartite RNA sequence. EMSAs with the selected SRE-4 with decreasing concentrations of recombinant His-SLM-2 (A) and the SAM68 GSG domain (B) (by a factor of 2 from 1 μM) or with buffer alone. The RNA sequence and mutants (m1-m4) used in the reaction are shown in Table 2. Migration patterns of unbound RNAs (free probe) and protein bound RNAs (protein-RNA complex) are indicated on the left.
