Figure 3.

Defining the high affinity QRE within the MBPmRNA. (A) EMSAs of selected RNAs with increasing concentrations of recombinant GST-QKI-5 (by a factor of 2 from 2 nM) or with buffer alone. The RNAs used for the EMSAs are the MBP QRE-2 and variation mutants of each (m1-m6). Migration patterns of unbound RNAs (free probe) and QKI-5 bound RNAs (QKI-RNA complex) are indicated on the left. (B) RNA species tested in (A) are shown. The black bars denote sequences that are unaltered between the wild-type and the mutant versions.