Table 3.
Details of qPCR and genotyping assay primers and conditions
| Gene | Assay on demand ID | Genotype variant | Genotype detection method | Genotyping primers (forward and reverse) | Size (bp) |
Temp °C |
MgCl2 (mM) |
| TPH1 | Hs00188220_m1 | T-346G |
MslI enzyme digest |
5'CTTCGTTATGTGTACAGTCC-3' 5'TAGGACTGCAGTGCTTCTC-3' |
G = 365 T = 305+60 |
59 | 2 |
| TPH2 | Hs00542783_m1 | G-703T | Taqman allelic discrimination | 5'ACACTCACACATTTGCATGCAC-3' 5'CATTGACCAACTCCATTTTATGTTAATAAGCT-3' |
|||
|
TPH2 taqman genotyping probe sequences: VIC-CTTGACATATTCTAATTTT FAM-ACTTGACATATTATAATTTT |
|||||||
| 5-HT2A | Hs00167241_m1 | A-1438G | MspI enzyme digest | 5'AAGCTGCAAGGTAGCAACAGC-3' 5'AACCAACTTATTTCCTACCAC-3' |
A = 468 G = 244+224 |
56 | 1.5 |
| 5-HT2C | Hs00168365_m1 | GTn promoter microsatellite -1027 bp |
capillary electrophoresis | FAM5'GGGAGTTTCAAAGCTTGATGA3' 5'GTTTCTTAGACCCATGGTGGAGATGG-3' |
~259; Z-6 allele |
59.5 | 2.5 |
| 5-HTT | Hs00169010_m1 | 5-HTTLPR | agarose gel electrophoresis | 5'ATGCCAGCACCTAACCCCTAATGT-3' 5'GGACCGCAAGGTGGGCGGGA-3' |
419; 16 rpt 'L' |
66 | 1.5 |
| intron2 VNTR | agarose gel electrophoresis | 5'GTCAGTATCACAGGCTGCGAG-3' 5'TGTTCCTAGTCTTACGCCAGT-3' |
STin12 ~299 | 54 | 1.5 | ||
| GAPDH | Hs00266705_g1 | n/a | |||||
| UBC | Hs00824723_m1 | n/a | |||||
| SDHA | Hs00188166_m1 | n/a |
Assay-on-demand' IDs correspond to product order numbers (Applied Biosystems).