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. 2000 Jul 11;97(15):8519–8524. doi: 10.1073/pnas.140217197

Figure 2.

Figure 2

Effect of tRA on NIS mRNA level in MCF-7 cells. (a) Time course of tRA-induced up-regulation of NIS mRNA expression in MCF-7 cells. Cells were incubated with 1 μM tRA for indicated time and then Northern blot analysis was performed with 25 μg of total RNA. Radioactivity of the blots was quantitated, and the intensity of NIS mRNA was normalized to β-actin mRNA (Lower). Values are mean ± SD (n = 3). (b) Dose dependency of tRA on NIS mRNA induction in MCF-7 cells. Cells were incubated with indicated concentration of tRA for 12 h and then Northern blot analysis with 25 μg of total RNA was performed. These experiments are representative of three experiments performed with similar results.