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. 2000 Jul 11;97(15):8519–8524. doi: 10.1073/pnas.140217197

Figure 6.

Figure 6

Iodide efflux is reduced in MCF-7 cells compared with FRTL-5 rat thyroid cells. (a) Iodide efflux from tRA-treated MCF-7 cells compared with that from FRTL-5 rat thyroid cells. MCF-7 cells were treated with 1 μM tRA for 48 h before the assay. FRTL-5 cells were maintained with 1 milliunit/ml TSH. ●, MCF-7 cells; x, FRTL-5 cells. (b) Iodide organification in tRA-treated MCF-7 cells and FRTL-5 cells. Cells were incubated for 2 h at 37° C with 500 μM HBSS containing ≈1.0 μM Na125I and 100 μM NaI. Nonspecific binding of 125I- was determined in duplicate assays in the presence of 30 μM KClO4. The radioactivity of trichloroacetic acid-precipitate was normalized to the cellular protein content measured in the same cells. Data are means ± SD (n = 3).