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. Author manuscript; available in PMC: 2009 Jun 18.
Published in final edited form as: Mol Microbiol. 2009 Apr 30;72(5):1087–1099. doi: 10.1111/j.1365-2958.2009.06712.x

FIG. 2.

FIG. 2

Electrophoretic mobility shift assay (EMSA) on T. pallidum tprE and tprG (Panel A), tprJ and lac (Panel B) promoters in presence of cAMP and increasing concentrations (0μM, 10 μM, 20μM, and 30 μM, respectively) of recombinant TP0262 (lanes 1-4 for the tpr genes) or E. coli CRP (lanes 6-9 for the E. coli lac promoter) proteins. TP0262 + cAMP bound the T. pallidum tpr promoters; E. coli CRP + cAMP failed to bind tpr promoters in this assay (data not shown). Recombinant E. coli CRP was shown to retard electrophoretic mobility of the lac promoter amplicon containing the TGTGA-6N-TCACA binding site. Without cAMP (lines 5 and 10), no binding was seen. 0.5 fmols of labeled probe were used in each experiment.