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. Author manuscript; available in PMC: 2009 Jun 18.
Published in final edited form as: Mol Microbiol. 2009 Apr 30;72(5):1087–1099. doi: 10.1111/j.1365-2958.2009.06712.x

FIG. 4.

FIG. 4

Recombinant TP0262 binds the DNA sequences identified by DNaseI footprinting assay. In presence of unlabelled competitor oligonucleotide fragments (Table II) containing the TP0262 tprE, tprG (Panel A), and J (Panel B) binding sites identified by footprinting assay, no shift of the labeled amplicons (containing the putative TP0262 binding sites) used in the previous EMSA experiments was observed. As positive control, a 22 bp unlabelled oligonucleotide (Table II) containing the E. coli CRP binding site prevented E. coli CRP from shifting the labeled lac promoter amplicon. In each experiment, the amount of the unlabelled fragments was 100 fold higher (50 fmol) than the labeled probe (0.5 fmol).