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cAMP is required for TP0262 influence tprJ promoter activity. The constructs containing the tprJ promoters were used to transform a crp and cya-deficient E. coli strain (G1044, Δcrp::cm::cya-, unable to synthesize cAMP). The positive regulatory effect exerted by TP0262 on the tprJ promoter (containing the region with the putative CRP binding site) was absent in culture media not supplemented with cAMP.
