Figure 3.

Determination of the corin cleavage site in pro-ANP. Expression vectors for wild-type pro-ANP or mutant pro-ANPs R98G, R101A, and R102A were cotransfected into 293 cells with corin expression vector, pcDNACorin. Pro-ANP and its derivatives in conditioned medium were analyzed by Western blotting using an anti-V5 antibody. At high resolution, two specific bands of pro-ANP were detected on the Western blot possibly caused by differences in glycosylation in transiently transfected 293 cells.