Figure 10. Loss of TG accumulation in Δ-tgs1 (Δ-Rv3130c) under multiple-stress (18 days) and its restoration by complementation.
Equal amount of lipid for each strain was loaded to Silica-TLC and resolved using hexane-diethyl ether-formic acid (90∶10∶1, v/v/v) solvent system. Lipids were visualized by charring for 10 min at 180°C after spraying with dichromate-sulfuric acid. WE, wax ester; TG, triglyceride; WT-H37Rv, Mtb H37Rv; Δ-tgs1, tgs1 deletion mutant of Mtb H37Rv; C-Δ-tgs1, tgs1 complemented strain of Δ-tgs1 mutant.