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. 2009 Jun 29;4(6):e6077. doi: 10.1371/journal.pone.0006077

Figure 11. (A), Diminished ‘loss of acid-fastness’ in the Δ-tgs1 mutant population under multiple-stress.

Figure 11

WT-H37Rv, Δ-tgs1 mutant and C-Δ-tgs1 cells were stained with Auramine-O (acid-fast) and Nile Red (neutral lipid) after 18 days under multiple-stress. Bar = 4 µM. (B), Percent acid-fast stain positive cells observed in different Mtb strains under multiple-stress for 9 and 18 days. WT-H37Rv, wild type Mtb H37RV; Δ-tgs1, tgs1 deletion mutant of WT-H37Rv; C-Δ-tgs1, tgs1 complemented strain of Δ-tgs1.