Table 3. Comparison of Antibiotic Resistance Evaluation by Alamar Blue and Agar Plating Methods.

Antibiotic	Percent Antibiotic Resistance in Mtb population after day 9 under multiple-stress
	Alamar Blue	Agar Plating Method
Rif, 0.1 µg/ml	65±8	100
Rif, 5.0 µg/ml	3±1	4±1
INH, 0.8 µg/ml	64±14	40±20

Cultures of Mtb at 9 day in multiple-stress were treated with antibiotic for 5 days. Viable cells surviving the antibiotic treatment were allowed to grow for 5 days by 200-fold dilution into media without antibiotic. The viable cells were quantified using Alamar Blue dye as described in Materials and Methods.