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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: J Biomol Screen. 2009 Feb 4;14(2):161–172. doi: 10.1177/1087057108328761

Fig. 6. Compound Selectivity for LARG-stimulated RhoA [35S] GTPγS Binding.

Fig. 6

(A) Time-course of RhoA (C189S) (■) (500 nM) [35S] GTPγS binding over 180 minutes. (B) Time-course of Gαo (●) (250 nM) [35S] GTPγS binding over 120 minutes. Both no RhoA and no Gαo or [35S] GTPγS alone are represented by (■). (C) Bar graph representation of compound (100 μM) selectivity for LARG-stimulated [35S] GTPγS binding versus RhoA (C189S) and Gαo [35S] GTPγS binding. Binding was measured at a 60 minute time point (indicated by the dotted vertical line) for RhoA (C189S) and 25 minute time point (indicated by the dotted vertical line and open circles) for both Gαo and LARG-stimulated RhoA [35S] GTPγS binding. Data in panel A, B, and C were measured in duplicate and represent mean ± SEM for three separate experiments (N = 3). Data in panel C is represented as percent of DMSO control for [35S] GTPγS binding.