Figure 5.

Binding activity of inactive CS mutants in the presence of heparin or heparinase. (A) 50 nM of inactive mutants was coincubated with either 5 nM of heparin (shaded bars) or with buffer alone (black bars) for 15 min followed by their addition onto the cells. Fluorescence was measured as described. (B) HepG2 cells were preincubated with heparinase (shaded bars) or with buffer (black bars) for 2 h followed by addition of 50 nM of CS15 coincubated with buffer or with 5 nM of heparin. Fluorescence was measured as described.