Figure 4. High throughput screening for systematic study on molecular mechanisms underlying the pMV-absorption.

(A) Flow diagram of the high throughput screening (HTS) for isolation of small molecules modulating the pMV-absorption. (B) Z-factor was calculated with averages and standard deviations of 32 sets of assays; each set included assays with control mAb plus QL9-loaded pMVs used as the positive control, anti-LFA-1 mAb plus QL9-loaded pMVs used as the negative control and with control mAb plus P1A-loaded pMVs. (C) IC₅₀s (or ED₅₀) and pharmacological uses of hits isolated from the pilot scale HTS were summarized.