Rhodamine phalloidin-stained cells. Row A, DXA-CT-GFP cells continuously produce large amounts of CT-GFP protein and form Hirano bodies. Row B, cells containing the pRNR-CT-GFP sequence show inclusions, which colocalize with F-actin and GFP. Row C, cell lines expressing the CT sequence alone from the RNR promoter show inclusions visible after phalloidin staining. UV light exposure did not have a discernible effect on AX2 cells. All cell lines, including AX2 (row D) wild-type populations, show the presence of F-actin in the cell cortex and other regions of dynamic actin activity. (Scale bar, 10 μm.) Images were taken at 24 h after promoter induction.