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. 2000 Jul 18;97(15):8536–8541. doi: 10.1073/pnas.97.15.8536

Table 1.

Primers employed

Gene Sense primer (5′–3′) Antisense primer (5′–3′) Predicted size, bp Species GenBank accession no.
RT-PCR primers employed for Northern probes
 ACC ACTCCAGGACAGCACAGATC TCTGCCAGTCCAATTCTAGC 535 Rat J03808
 FAS AGCCCAGAGGGATCTGGTGA TACACTCACTCGAGGCTCAG 1,158 Rat M76767
 GPAT TGATCAGCCAGGAGCAGCTG AGACAGTATGTGGCACTCTC 504 Rat AF021348
Primers for Multiplex RT-PCR
 SREBP-1a ACACAGCGGTTTTGAACGACATC ACGGACGGGTACATCTTTACAG 268 Rat, mouse L16995 (see ref. 21)
 SREBP-1c GGAGCCATGGATTGCACATT AGGAAGGCTTCCAGAGAGGA 191 Rat L16995
 SREBP-2 CACAATATCATTGAAAAGCGCTACC TTTTTCTGATTGGCCAGCTTCAGCA 200 Mouse (see ref. 21)
  ACC ACAGTGAAGGCTTACGTCTG AGGATCCTTACAACCTCTGC 242 Rat J03808
  FAS TGCTGTGGACCTCATCACTA TGGATGATGTTGATGATAGAC 297 Rat M76767
  GPAT CCTCTGAACTGGAGAAGTGA AGACAGTATGTGGCACTCTC 287 Rat AF021348
  TBP ACCCTTCACCAATGACTCCTATG TGACTGCAGCAAATCGCTTGG 186 Mouse D01034