Table 1.
Gene | Sense primer (5′–3′) | Antisense primer (5′–3′) | Predicted size, bp | Species | GenBank accession no. |
---|---|---|---|---|---|
RT-PCR primers employed for Northern probes | |||||
ACC | ACTCCAGGACAGCACAGATC | TCTGCCAGTCCAATTCTAGC | 535 | Rat | J03808 |
FAS | AGCCCAGAGGGATCTGGTGA | TACACTCACTCGAGGCTCAG | 1,158 | Rat | M76767 |
GPAT | TGATCAGCCAGGAGCAGCTG | AGACAGTATGTGGCACTCTC | 504 | Rat | AF021348 |
Primers for Multiplex RT-PCR | |||||
SREBP-1a | ACACAGCGGTTTTGAACGACATC | ACGGACGGGTACATCTTTACAG | 268 | Rat, mouse | L16995 (see ref. 21) |
SREBP-1c | GGAGCCATGGATTGCACATT | AGGAAGGCTTCCAGAGAGGA | 191 | Rat | L16995 |
SREBP-2 | CACAATATCATTGAAAAGCGCTACC | TTTTTCTGATTGGCCAGCTTCAGCA | 200 | Mouse | (see ref. 21) |
ACC | ACAGTGAAGGCTTACGTCTG | AGGATCCTTACAACCTCTGC | 242 | Rat | J03808 |
FAS | TGCTGTGGACCTCATCACTA | TGGATGATGTTGATGATAGAC | 297 | Rat | M76767 |
GPAT | CCTCTGAACTGGAGAAGTGA | AGACAGTATGTGGCACTCTC | 287 | Rat | AF021348 |
TBP | ACCCTTCACCAATGACTCCTATG | TGACTGCAGCAAATCGCTTGG | 186 | Mouse | D01034 |