TABLE 1.
Primers used
| Primera | Sequence |
|---|---|
| 5318DNF1* | 5′AAA AGG TCG ATG AGT AAG GAA ATG3′ |
| 5318DNR2* | 5′CAA TCT CAG GGT ACG CGG3′ |
| Lumpy 2 | 5′CAU CAU CAU CAU CCC CAA GCA GAA TCG TTG3′ |
| Lumpy 3 | 5′CAU CAU CAU CAU GTT ACC ACC GAG TTG TGG G3′ |
| Lumpy 6 | 5′CAU CAU CAU CAU CAC AAC TCG GTG GTA ACA AC3′ |
| Lumpy 7 | 5′GTT GTG GAG AAT TGC ATA C3′ |
| Lumpy 8 | 5′CAU CAU CAU CAU GGT TAT TTG CTG CTC TTG C3′ |
| Lumpy 9 | 5′CGA TAC CGC TCC CCA ATA TG3′ |
| Lumpy 10 | 5′CTA TAT TCT TGT CTA CAT TAG TC3′ |
| Lumpy 11-GFP | 5′ACC TCG AGA TGT GTT TTT TTC ATA ATT AAC CAT CAT ATT GG3′ |
| Lumpy 12-GFP | 5′TGG GCC CTC AAA ACA AAC TGA TAG AGT TTT AAT AAA TGC3′ |
| Lumpy 13 | 5′ACC TCG AGA TGC AAT ACG AAC AAC TAA ACC AA3′ |
| Lumpy 14 | 5′TGG GCC CTC ATA ATT AAC CAT CAT ATT GGG GAG C3′ |
| DR377** | 5′CAA AAC AAA CTG ATA GAG3′ |
| DR378** | 5′TGT TAC CAC CGA GTT GTG3′ |
| P1(+)** | TGT TAC CAC CGA GTT GTG |
| P1(−)** | CAA AAC AAA CTG ATA GAG |
| DR550** | 5′CGC ATT TAG AAGCTC TCT CAG GAT T3′ |
a
All Lumpy primers were synthesized by Invitrogen. *, these primers were a gift from A. Turkewitz, University of Chicago. **, these primers were synthesized at the University of Minnesota.