FIG. 3.
Altered peroxisome profiles and ultrastructure in cells repressed for SEC20, SEC39, or DSL1. (A to F) Strains R1158 (A), THC-SEC14 (B), THC-SEC61 (C), THC-SEC20 (D), THC-SEC39 (E), and THC-DSL1 (F) were incubated for 16 h in YEPD prior to incubation in YPBO for 7 h. (A′ to F′) Repression of TetO7 promoter-regulated genes was achieved by addition of doxycycline to a final concentration of 10 μg/ml to the medium. (D″ to F″) Increased magnification of selected areas from panels D′ to F′, respectively, to allow better visualization of the expanded vesicular regions associated with the ER (asterisks) resulting from repression of SEC20, SEC39, or DSL1. M, mitochondrion; N, nucleus; P, peroxisome. Bars, 1 μm in panels A to F′ and 0.5 μm in panels D″ to F″.