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Oleic acid induces the expression of Sec20p, Sec39p, and Dsl1p. Equal amounts of protein from whole-cell lysates of wild-type BY4742 cells cultured in oleic acid-containing SCIM1 for the times indicated were separated by SDS-PAGE, transferred to nitrocellulose, and immunoblotted for Sec20p, Sec39p, Dsl1p, the ER-resident protein Kar2p, and Pot1p.
