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Mutant M75 was disrupted in the FTL1 gene. (A) The transforming vector pKY37 was inserted into the EcoRI site (marked by an asterisk) of the FTL1 gene (empty arrow). Plasmids pKY12 and pKY25 were recovered from M75 by self-ligation with PstI- and KpnI-digested genomic DNA, respectively. B, BamHI; E, EcoRI; K, KpnI; P, PstI. (B) The FTL1 gene contains one N-terminal LisH domain (empty box) and WD40 repeats (solid box) at the C-terminal half of the protein.
