Table 1.
Lower limits of detection and quantification of various phospholipid and sterol species (n = 3)
| CuSO4 detection method | Nile Red detection method | |||
|---|---|---|---|---|
| LLDa (pmol) | LLQb (pmol) | LLD (pmol) | LLQ (pmol) | |
| Lipid species | ||||
| SM | 75.4 ± 35.1 | 171.4 ± 34.3 | 637.8 ± 373.7 | 823.0 ± 379.0 |
| DAPC | 93.8 ± 12.6 | 108.1 ± 39.3 | 655.1 ± 339.8 | 966.7 ± 392.8 |
| DAPI (bovine brain) | 85.6 ± 35.7 | 264.7 ± 90.3 | 526.0 ± 310.5 | 715.8 ± 258.8 |
| DAPS | 99.1 ± 36.8 | 374.1 ± 112.5 | 618.5 ± 323.1 | 1271.5 ± 361.8 |
| DAPE | 63.2 ± 34.7 | 194.9 ± 64.1 | 216.4 ± 60.4 | 470.4 ± 82.3 |
| DAPG | 69.0 ± 30.5 | 156.8 ± 50.6 | 214.5 ± 109.1 | 414.0 ± 90.0 |
| DAPA | 73.3 ± 34.5 | 207.5 ± 71.4 | 368.3 ± 167.2 | 1262.2 ± 428.8 |
| Average | 79.9 ± 11.5 | 211.1 ± 34.3 | 462.4 ± 99.2 | 846.2 ± 122.6 |
| Sterol species | ||||
| Stigmasterol | 6.3 ± 11 × 10−3 | 3.9 ± 1.1 | N.D. | N.D. |
| Lanosterol | 2.6 ± 1.9 × 10−2 | 15.6 ± 6.4 | 72.6 ± 18.7 | 515.6 ± 194.7 |
| Cholesterol | 9.7 ± 3.2 × 10−2 | 6.5 ± 1.5 | 45.3 ± 12.4 | 646.6 ± 0 |
| Average | 4.3 ± 1.6 × 10−2 | 8.7 ± 2.9 | 59.0 ± 12.3 | 581.1 ± 109.8 |
aThree standard deviations above background
bTen standard deviations above background