TABLE 2.

Primers^a

Primer	Sequence (5′-3′)	Purpose
8RT-R	CACTGGACGACCAAGATCAA	RT-PCR; 5′ RACE
8RT-F	AGCAGGTAGTCCGACAGGTG	RT-PCR; 3′ RACE
8GSP2	CGTTGCTGGTGAACAAGAGC	5′ RACE
8GSP3	GTCTGCTGCCGCGGATCAT	5′ RACE
8GSP5	GACGTCTTCGGAATCAAGC	3′ RACE
ppk-UIC-FWD	GGGagatctGCATAACTGCCGAAGAACGC	Construction of ppk-lacZ
ppk-UIC-REV	GGGactagtTACAGGCTGCTGTCATCCAG	Construction of ppk-lacZ
8D5F	CAATCGCTCCAGCAACGTCG	SOE-PCR deletion of iiv8 and ppk; PCR to construct pSR-8DFR
8D5R	CCGTGCCGCTCAATTGTTAGCGCCGTTCACCCTGAAGAAG	SOE-PCR deletion of iiv8 and ppk
8D3F	CTTCTTCAGGGTGAACGGCGCTAACAATTGAGCGGCACGG	SOE-PCR deletion of iiv8 and ppk
8D3R	ACATCACCGTCGACGCACTG	SOE-PCR deletion of iiv8 and ppk; PCR to construct pSR-8DFR
8D5F2	GACCGTGCATTGAGTAACG	Confirming deletion of iiv8 and ppk
8D3R2	GCGTGAGAATGTGTTGACG	Confirming deletion of iiv8 and ppk
8compF	GCGaagcttCAGCAGCTTCTTGCCTTC	Construction of pME8comp
8compR	GCGaagcttCTTCGTCTATCAGGTCAG	Construction of pME8comp
8D5F2H	GGGaagcttGACCGTGCATTGAGTAACG	Construction of pMEppk
hemF-H	GGGaagcttGTCATGCAGAAATTGTTGG	Construction of pMEppk

^aRestriction sites engineered into the primers are shown in lowercase letters.