TABLE 2.
VNTR loci found with Tandem Repeat Finder and microorganism tandem repeat database
Locus | Physical position (kb) | Chromosome | Unit length (bp) | Copy no. | Total length (bp) | % Matches | % C | % G | % T | % A | GC bias | Commentsa |
---|---|---|---|---|---|---|---|---|---|---|---|---|
VP1-01 | 384 | 1 | 101 | 4.3 | 433 | 81 | 15 | 26 | 30 | 26 | 0.26 | Not amplified in all strains |
VP1-02 | 447 | 1 | 6 | 9.5 | 55 | 94 | 16 | 33 | 50 | 0 | 0.34 | Not tested |
VP1-03 | 452 | 1 | 24 | 3 | 72 | 91 | 22 | 33 | 26 | 18 | 0.19 | Not tested |
VP1-04 | 772 | 1 | 480 | 3.2 | 1540 | 83 | 24 | 22 | 24 | 27 | 0.04 | Not tested* |
VP1-05 | 978 | 1 | 166 | 5.8 | 967 | 98 | 23 | 25 | 25 | 25 | 0.04 | Not tested* |
VP1-06 | 1478 | 1 | 576 | 6.5 | 3743 | 90 | 25 | 28 | 17 | 27 | 0.05 | Not tested* |
VP1-07 | 1648 | 1 | 33 | 2 | 64 | 81 | 28 | 25 | 14 | 31 | 0.03 | Not tested |
VP1-08 | 1662 | 1 | 21 | 2.7 | 55 | 80 | 35 | 17 | 8 | 37 | 0.34 | Not amplified in all strains |
VP1-09 | 1730 | 1 | 366 | 12 | 4395 | 90 | 26 | 25 | 25 | 22 | 0.01 | Not tested* |
VP1-10 | 2213 | 1 | 114 | 6.8 | 772 | 95 | 26 | 24 | 15 | 33 | 0.03 | Variable in size |
VP1-11 | 2301 | 1 | 129 | 5.8 | 751 | 85 | 28 | 23 | 30 | 17 | 0.09 | Variable in size |
VP1-12 | 2339 | 1 | 6 | 21.5 | 131 | 89 | 31 | 31 | 34 | 3 | 0 | Variable in size |
VP1-13 | 2669 | 1 | 7 | 11.1 | 77 | 100 | 14 | 28 | 42 | 15 | 0.33 | Not amplified in all strains |
VP1-14 | 2989 | 1 | 238 | 2.1 | 506 | 95 | 25 | 24 | 24 | 26 | 0.02 | Not amplified in all strains |
VP1-15 | 3065 | 1 | 90 | 1.9 | 173 | 80 | 30 | 28 | 19 | 22 | 0.03 | Not tested |
VP1-16 | 3077 | 1 | 6 | 28.2 | 168 | 100 | 0 | 33 | 16 | 50 | 0.99 | Variable in size |
VP1-17 | 3249 | 1 | 30 | 1.9 | 57 | 82 | 22 | 17 | 12 | 47 | 0.12 | Invariable in size |
VP2-01 | 477 | 2 | 561 | 2 | 1125 | 99 | 19 | 22 | 34 | 23 | 0.07 | Not tested* |
VP2-02 | 990 | 2 | 306 | 14.1 | 4325 | 96 | 29 | 23 | 26 | 19 | 0.11 | Not tested* |
VP2-03 | 1292 | 2 | 26 | 2.2 | 56 | 80 | 12 | 14 | 39 | 33 | 0.07 | Invariable in size |
VP2-04 | 1341 | 2 | 8 | 22.8 | 181 | 100 | 25 | 12 | 12 | 49 | 0.35 | Not amplified in all strains |
VP2-05 | 1417 | 2 | 27 | 2 | 55 | 85 | 25 | 5 | 34 | 34 | 0.06 | Not amplified in all strains |
VP2-06 | 1428 | 2 | 960 | 2 | 1901 | 96 | 32 | 26 | 26 | 14 | 0.1 | Not tested* |
VP2-07 | 1548 | 2 | 6 | 35.2 | 207 | 94 | 32 | 17 | 0 | 50 | 0.3 | Variable in size |
Results of the 18 V. parahaemolyticus strains tested are summarized under Comments. *, not tested further because the target for the PCR was too long and no acceptable primers were found.