FIG. 4.

Kinetics of the expression of DAPG (A and C) and PLT (B and D) biosynthetic genes in growing cultures of P. fluorescens CHA0 (▪) and its Δgcd mutant, CHA1196 (○), both carrying either a phlA-gfp reporter fusion on pME7100 (A and C) or a pltA-gfp fusion on pME7109 (B and D). Gene expression is shown as relative fluorescence units (RFU), reflecting green fluorescence per bacterial density. The strains were grown in OS glucose (A and B) or OS gluconate (C and D) medium at 30°C. The data represent the means (± standard errors) of six replicate cultures. The experiment was repeated twice with similar results.