TABLE 2.
Production of the antifungal compounds DAPG and PLT by P. fluorescens CHA0 and its Δgcd and Δgad mutants
| Culture medium | Incubation time (days) | DAPG production (μM)a
|
PLT production (μM)a
|
||||
|---|---|---|---|---|---|---|---|
| CHA0 (wild type) | CHA1196 (Δgcd) | CHA1197 (Δgad) | CHA0 (wild type) | CHA1196 (Δgcd) | CHA1197 (Δgad) | ||
| YM glucose | 1 | 5.3 ± 1.2 | 118.1 ± 47.2 | 6.6 ± 1.2 | NDb | 7.5 ± 3.1 | ND |
| 2 | 51.7 ± 2.7 | 94.7 ± 28.2 | 37.7 ± 14.6 | ND | 11.9 ± 3.2 | ND | |
| 3 | 73.7 ± 9.8 | 113.3 ± 35.7 | 72.5 ± 13.8 | ND | 7.2 ± 1.4 | ND | |
| YM gluconate | 1 | ND | 40.9 ± 9.1 | 4.3 ± 5.3 | ND | ND | ND |
| 2 | 66.9 ± 13.5 | 62.1 ± 15.2 | 80.3 ± 4.9 | ND | ND | ND | |
| 3 | 18.2 ± 3.6 | 15.5 ± 1.6 | 12.0 ± 0.2 | ND | ND | ND | |
a
P. fluorescens wild-type CHA0 and its derivatives, CHA1196 (Δgcd) and CHA1197 (Δgad), were cultivated in 100 ml YM medium amended with 25 mM glucose or 25 mM gluconate and extracted with ethyl acetate. DAPG and PLT contents in extracts were quantified by high-performance liquid chromatography. The data represent the means (± standard errors) of three replicate cultures per treatment.
b
ND, not detected.