TABLE 3.
Growth inhibition of different plant-pathogenic fungi by P. fluorescens CHA0 and its Δgcd and Δgad mutants
| Fungusa | Radial growth of fungal colony (radius in mm)b
|
|||
|---|---|---|---|---|
| No bacteria added | CHA0 (wild type) | CHA1196 (Δgcd) | CHA1197 (Δgad) | |
| G. graminis var. tritici | 29a | 8b | 5c | 7b |
| M. grisea | 17a | 12b | 9c | 13b |
| R. solani | 43a | 26b | 24b | 25b |
G. graminis var. tritici, M. grisea, and R. solani were inoculated in the center of a one-fifth-strength PDA plate. P. fluorescens wild-type CHA0 and its derivatives, CHA1196 (Δgcd) and CHA1197 (Δgad), were streaked out in a square of 5.5-cm lateral length around the fungal plugs and grown for 5 days at 24°C.
The data are means of eight replicates. Means for the same fungus within the same row followed by different letters are significantly different at a P value of ≤0.05 according to Fisher's least significant difference test. The experiment was repeated twice with similar results.