TABLE 2.
Plasmids used in this study
| Plasmid name | Description | Source or reference |
|---|---|---|
| pEGFPN1 | GFP expression vector; Ap | BD Biotech |
| pGEM-T Easy | Cloning vector; Ap | Promega |
| pBBR1MCS-2 | Mobilizable broad-host-range vector; Km | 8 |
| pGEMegfp | pGEM-T Easy + egfp PCR product | This study |
| pBBRegfp | pBBR1MCS-2 + egfp from pGEMegfp | This study |
| pBBRpl | Promoterless GFP reporter vector based on pBBRegfp | This study |
| pGEMPmamDC | pGEM-T Easy + PmamDC-PCR product | This study |
| pGEMPmamAB | pGEM-T Easy + PmamAB-PCR product | This study |
| pGEMPapdA | pGEM-T Easy + PapdA-PCR product | This study |
| pGEMPmsp3 | pGEM-T Easy + Pmsp3-PCR product | This study |
| pGEMPrplK | pGEM-T Easy + PrplK-PCR product | This study |
| pGEMPrpsJ | pGEM-T Easy + PrpsJ-PCR product | This study |
| pBBRPmamDC | pBBRpl + PmamDC from pGEMPmamDC cloned in XhoI and NdeI sites | This study |
| pBBRPmamAB | pBBRpl + PmamAB from pGEMPmamAB cloned in XhoI and NdeI sites | This study |
| pBBRPapdA | pBBRpl + PapdA from pGEMPapdA cloned in XhoI and NdeI sites | This study |
| pBBRPmsp3 | pBBRpl + Pmsp3 from pGEMPmsp3 cloned in XhoI and VspI sites | This study |
| pBBRPrplK | pBBRpl + PrplK from pGEMPrplK cloned in HindIII and NdeI sites | This study |
| pBBRPrpsJ | pBBRpl + PrpsJ from pGEMPrpsJ cloned in XhoI and NdeI sites | This study |
| pEZZ18 | Protein A gene fusion vector; Ap | GE Healthcare |
| pGEMZZ | pGEM-T Easy + ZZ protein domain PCR product | This study |
| pCL6 | pBBR1MCS-2 + MamC-GFP | 9 |
| pBBRCZZ | The ZZ protein domain from pGEMZZ was cloned into the NdeI and BamHI sites of pCL6 to replace GFP with the ZZ domain | This study |
| pJETPdcx1 | pJET1.2 + PmamDC-PCR product | This study |
| pBBRPdcCZZ | pBBRCZZ + PmamDC from pGEMPdcx1 | This study |