FIG. 5.

Analysis of E. faecalis JH2-2 transconjugants. (A) (GTG)₅ PCR fingerprinting patterns of independent E. faecalis transconjugants (lanes 1 to 3), the L. paracasei donor strain (D), and the E. faecalis JH2-2 recipient strain (R). (B) PCR amplification of the Tn916 circular intermediate with primer pair IV (Table 1). Results are shown for the L. paracasei donor strain (D), the independent transconjugants (lanes 1 and 2), the E. faecalis JH2-2 recipient strain (R), and the negative control lacking template DNA (−).