TABLE 1.
Primers used in PCR amplifications
| Primer pair | Name | Sequence | Annealing temp (°C) | Amplicon size (bp) | Source or reference |
|---|---|---|---|---|---|
| I | tetM-1 | GAACTCGAACAAGAGGAAAGC | 60 | 740 | 21 |
| tetM-2 | ATG GAAGCCCAGAAAGGAT | ||||
| II | Int-FW | GCGTGATTGTATCTCACT | 50 | 1,028 | 12 |
| Int-RV | GACGCTCCTGTTGCTTCT | ||||
| III | Tet-int-FW | CGGATAGATAAAGTACGATA | 52 | 2,659 | This study |
| Tet-int-RV | TCACGTCTTTTTTCTGACAT | ||||
| IV | For-ext | CATTCACATCGAAGTGCCGCCAAATCC | 65 | 17,354 | This study |
| Rev-ext | GCTTTCCTCTTGTTCGAGTTCCAATGC | ||||
| V | nt 18K-FW | GCA AATGCAGGAATGAATCCAAAGG | 60 | 13,042 | This study |
| Rev-ext | GCTTTCCTCTTGTTCGAGTTCCAATGC | ||||
| VI | int-tag-FW | AGAAGCAACAGGAGCGTC | 52 | 473 | This study |
| nt288-RV | GCTGAATGAATGTTTGATGG |