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. 2009 Apr 17;75(12):4015–4027. doi: 10.1128/AEM.02733-08

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Copyright © 2009, American Society for Microbiology

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FIG. 2. — The mini-Tn7-Tel^r integration vector and its pTNS3-asd_Ec helper plasmid. (A) mini-Tn7-Tel^r, a Tn7-based suicidal vector with the Tel^r marker. (B) pTNS3-asd_Ec suicidal helper plasmid encoding the transposase, which catalyzes Tn7 transposition. (C) Chromosomally inserted mini-Tn7-Tel^r elements at two different B. thailandensis attTn7 sites as previously described (6). Oligonucleotides 874, 875, and 876, indicated by arrows, were used to screen for the location of transposition. glmS1 and glmS2 encode glucosamine-6-phosphate synthetases; MCS, multiple-cloning site; P1, P1 integron promoter; Tn7L, Tn7 transposase left recognition sequence; Tn7R, Tn7 transposase right recognition sequence; T₀T₁, transcriptional terminators; tnsABCD, Tn7 transposases.