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. 2009 Apr 17;75(12):4015–4027. doi: 10.1128/AEM.02733-08

FIG. 3.

FIG. 3.

Growth analyses of the B. thailandensis ΔbetBA mutant and its complement on choline and glucose. Wild-type strain E264, the E264-ΔbetBA::FRT/attTn7::Telr mutant, and the E264-ΔbetBA::FRT/attTn7::Telr-betBA complement were grown in 1× M9 minimal medium supplemented with 30 mM choline (A) or 20 mM glucose (B). All three strains exhibited similar growth rates and overall cell densities when grown in glucose. The complemented strain, E264-ΔbetBA::FRT/attTn7::Telr-betBA, displayed the same growth rate as the wild-type (wt) strain on choline. However, the betBA mutant strain containing the empty Tn7 transposon control, E264-ΔbetBA::FRT/attTn7::Telr, was not able to grow on choline as a sole carbon source. OD600, optical density at 600 nm.