TABLE 1.
Bacterial strains utilized in this study
| Strain | Lab IDa | Relevant characteristic(s) | Source or reference |
|---|---|---|---|
| E. coli | |||
| EPMax10B-pir116 | E1249 | F− λ−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu)7697 galU galK rpsL nupG Tn-pir116-FRT2 | Laboratory collection |
| DH5α-pir | E0175 | Tcr; F− λ− φ80dlacZΔM15 (lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 thi-1 gyrA96 relA1 uidA::pir zdg-232::Tn10 | 31 |
| HPS1-mob-Δasd-pir | E0463 | Tcr Kmr Cmr; e14− (mcrA) recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 Δ(lac-proAB) rif zxx::mini-Tn5-Lac4 (lacIq+lacZ M15) Δasd::FRT uidA::pir zdg-232::Tn10 recA::RP4-2 Tc::Mu Kmr | —b |
| EPMax10B-pir116-Δasd::Gmr | E1345 | Gmr; F− λ−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu)7697 galU galK rpsL nupG Tn-pir116-FRT2 Δasd::Gmr | —b |
| EPMax10B-pir116-Δasd-mob-Kmr-Δtrp::Gmr | E1354 | Kmr Gmr; F− λ−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu)7697 galU galK rpsL nupG Tn-pir116-FRT2 Δasd::FRT recA::RP4-2 Tc::Mu Kmr Δtrp::Gmr | —b |
| DH5α-λattB::pCD13SK-Flp | E0982 | Spr; F− φ80dlacZΔM15 (lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 thi-1 gyrA96 relA1 λattB::pCD13SK-Flp | —b |
| B. thailandensis | |||
| E264 | E1298 | Prototroph | 5 |
| E264-ΔbetBA::FRT | E1671 | B. thailandensis ΔbetBA::FRT mutant | 2 |
| E264-ΔbetBA::FRT/attTn7::Telr | E1709 | Telr; B. thailandensis ΔbetBA::FRT mutant with empty vector mini-Tn7-Telr integrated at the attTn7 site | This study |
| E264-ΔbetBA::FRT/attTn7::Telr-betBA | E1711 | Telr; B. thailandensis ΔbetBA::FRT mutant with mini-Tn7-Telr-betBA integrated at the attTn7 site | This study |
| E264-ΔbetBA::FRT-lacZ-Telr | E1731 | Telr; B. thailandensis ΔbetBA::FRT mutant with FRT-lacZ-Telr fusion | This study |
| E264-ΔbetBA::FRT-gfp-Telr | E2045 | Telr; B. thailandensis ΔbetBA::FRT mutant with FRT-gfp-Telr fusion | This study |
| E264-ΔbetBA::FRT-lux-Telr | E2047 | Telr; B. thailandensis ΔbetBA::FRT mutant with FRT-lux-Telr fusion | This study |
| E264-ΔbetBA::FRT-lacZ-Telr/attTn7::betBA | E1849 | Telr; E264-ΔbetBA::FRT/attTn7::betBA with ΔbetBA-lacZ-Telr fusion | This study |
| E264-ΔbetBA::FRT-gfp-Telr/attTn7::betBA | E2046 | Telr; E264-ΔbetBA::FRT/attTn7::betBA with ΔbetBA-gfp-Telr fusion | This study |
| E264-ΔbetBA::FRT-lux-Telr/attTn7::betBA | E2048 | Telr; E264-ΔbetBA::FRT/attTn7::betBA with ΔbetBA-lux-Telr fusion | This study |
| B. cenocepacia | |||
| K56-2 | E1554 | Prototroph; cystic fibrosis isolate | P. Sokol |
| J2315 | E1553 | Prototroph | J. Goldberg |
| B. pseudomallei | |||
| K96243 | B0005 | Prototroph; clinical isolate | 18 |
| 1026b | B0003 | Prototroph; clinical isolate | 11 |
| B. mallei ATCC 23344 | B0001 | Prototroph; clinical isolate | 40 |
a
For strains constructed in this study, please see the text for further details. Please use the laboratory identification (Lab ID) number to request strains.
b
—, details on the engineering of these strains will be published elsewhere.