TABLE 2.
Bacterial plasmids utilized in this study
| Plasmid | Lab IDa | Relevant properties | Reference or source |
|---|---|---|---|
| pBT20-Δbla | E1029 | Gmr; mariner transposon plasmid | 20 |
| pwFRT-PS12-Telr | E1584 | Telr; PS12-Telr cassette flanked by wild-type FRT sequences | 2 |
| pBT20-Δbla-Telr-FRT | E1727 | Telr; mariner transposon plasmid based on Telr | This study |
| pCD11-Gmr-pir116-oriT | E1254 | Cmr Gmr; conjugation vector | Laboratory collection |
| pUC18R6KT-mini-Tn7 | E1190 | Apr; Tn7-based broad-host-range transposon vector | 9 |
| mini-Tn7-Telr-bla | E1645 | Apr Telr; Telr cassette cloned into pUC18R6K-mini-Tn7 | This study |
| mini-Tn7-Telr | E1825 | Telr; mini-Tn7-bla-Telr with bla gene deleted | This study |
| mini-Tn7-Telr-betBA | E1829 | Telr; mini-Tn7-Telr with betBA operon cloned | This study |
| pTNS3 | E1189 | Apr; helper plasmid for Tn7 transposition system | 9 |
| pTNS3-asdEc | E1831 | pTNS3 with bla replaced by the E. coli asd gene | This study |
| pFRT1-lacZ | E0790 | Gmr; FRT1-lacZ fusion containing suicidal vector | 37 |
| pFRT1-lacZ-Telr | E1707 | Telr; pFRT1-lacZ with Gmr cassette replaced by Telr cassette | This study |
| pFRT2-lacZ | E0787 | Gmr; FRT2-lacZ fusion containing suicidal vector | 37 |
| pFRT2-lacZ-Telr | E1708 | Telr; pFRT2-lacZ with Gmr cassette replaced by Telr cassette | This study |
| pPS856-ΔXbas | E1044 | Gmr Apr; Gmr cassette flanked by wild-type FRT sequences | —b |
| pPS747 | E0042 | Apr; gfp-containing vector | 17 |
| pAKlux2 | E1863 | Apr; luxCDABE bioluminescence operon-containing vector | 21 |
| pFRT1-Gmr-lacZ-Telr | E2049 | Gmr Telr; pFRT1-lacZ-Telr with FRT1 replaced by the FRT1-Gmr-FRT1 fragment | This study |
| pFRT2-Gmr-lacZ-Telr | E2050 | Gmr Telr; pFRT1-lacZ-Telr with FRT1 replaced by the FRT2-Gmr-FRT2 fragment | This study |
| pFRT1-lacZ-Telr-ΔBam | E2051 | Telr; pFRT1-Gmr-lacZ-Telr with Flp-excised FRT1-Gmr | This study |
| pFRT2-lacZ-Telr-ΔBam | E2052 | Telr; pFRT2-Gmr-lacZ-Telr with Flp-excised FRT2-Gmr | This study |
| pFRT1-gfp-Telr | E2053 | Telr; pFRT1-lacZ-Telr-ΔBam with gfp replacing lacZ | This study |
| pFRT2-gfp-Telr | E2055 | Telr; pFRT2-lacZ-Telr-ΔBam with gfp replacing lacZ | This study |
| pFRT1-lux-Telr | E2064 | Telr; pFRT1-lacZ-Telr-ΔBam with luxCDABE replacing lacZ | This study |
| pFRT2-lux-Telr | E2066 | Telr; pFRT2-lacZ-Telr-ΔBam with luxCDABE replacing lacZ | This study |
| pCD13SK-Flp-oriT | E0803 | Spr; Flp-containing suicidal vector | 37 |
| pCD13SK-Flp-oriT-asdEc | E1827 | pCD13SK-Flp-oriT with asdEc replaced by the Spr cassette | This study |
| pFlpAB-5 | E1662 | Tpr; broad-host-range Flp-containing vector | 2 |
a
For plasmids constructed in this study, please see the text for further details. Please use the laboratory identification (Lab ID) number when requesting plasmids.
b
—, details on the engineering of this plasmid are to be published elsewhere.