TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| X. nematophila strains | ||
| HGB800 | X. nematophila ATCC 19061, wild type | American Type Culture Collection |
| HGB1227 | HGB800 Tn7 | 21 |
| HGB1230 | HGB800 ΔcpxR1::Str | 21 |
| HGB1231 | HGB800 ΔcpxR1::Str Tn7 | 21 |
| HGB1232 | HGB800 ΔcpxR1::Str Tn7-cpxRA | 21 |
| HGB1103 | HGB800 ΔnilR17::Kan | 5 |
| HGB1345 | HGB800 ΔnilR17::Kan ΔcpxR1::Str | This study |
| HGB1117 | HGB800 Tn7-nilA-lacZ | 5 |
| HGB1121 | HGB800 Tn7-nilB-lacZ | 5 |
| HGB1101 | HGB800 Tn7-nilC-lacZ | 4 |
| HGB1239 | HGB800 ΔcpxR1::Str Tn7-nilA-lacZ | 21 |
| HGB1240 | HGB800 ΔcpxR1::Str Tn7-nilB-lacZ | 21 |
| HGB1241 | HGB800 ΔcpxR1::Str Tn7-nilC-lacZ | 21 |
| HGB1119 | HGB800 ΔnilR17::Kan Tn7-nilA-lacZ | 5 |
| HGB1123 | HGB800 ΔnilR17::Kan Tn7-nilB-lacZ | 5 |
| HGB1346 | HGB800 ΔnilR17::Kan Tn7-nilC-lacZ | This study |
| HGB1347 | HGB800 ΔnilR17::Kan ΔcpxR1::Str Tn7-nilA-lacZ | This study |
| HGB1348 | HGB800 ΔnilR17::Kan ΔcpxR1::Str Tn7-nilB-lacZ | This study |
| HGB1349 | HGB800 ΔnilR17::Kan ΔcpxR1::Str Tn7-nilC-lacZ | This study |
| HGB1364 | HGB800 ΔmrxA1::Kan | This study |
| HGB1365 | HGB800 ΔcpxR1::Str ΔmrxA1::Kan | This study |
| E. coli S17-1(λpir) | Donor strain for conjugations | 47 |
| Plasmids | ||
| pPK13 | Allelic-exchange vector containing mrxA loci with aph cassette insertion | 3 |
| pTn7-nilA-lacZ | pEVS107+nilA 5′-flanking region fused to lacZ (Ermr Kanr) | 5 |
| pTn7-nilB-lacZ | pEVS107+nilB 5′-flanking region fused to lacZ (Ermr Kanr) | 5 |
| pTn7-nilC-lacZ | pEVS107+nilC 5′-flanking region fused to lacZ (Ermr Kanr) | 4 |
| pKRcpxRStr | Vector for allelic exchange of cpxR gene for aad, encoding streptomycin resistance | 21 |
a
Ermr, erythromycin resistance; Kanr, kanamycin resistance.