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SDS-PAGE gel of the LBOM protein fraction from Aurantimonas manganoxydans strain SI85-9A1 after successive purification by FPLC. Lane A, size standard; lane B, LBOM protein fraction; lane C, post-ion exchange chromatography; lane D, post-hydrophobic interaction chromatography; lane E, post-gel-filtration; lane F, size standard. The protein was silver stained with 1 μg total protein loaded into each well, except the size standard, for which 1 μl was used.
