TABLE 2.

Staining pattern for cell types in Dienes lines formed between strains 2 and 3^a

Time (h)b	Cell typec	% of cells
		Lived	Deadd	Dual staininge	No stainf
2	Rounded	81.25	6.25	12.5	0
2	Swarmer	94.25	5.75	0	0
2	Vegetative	97.5	2.5	0	0
4	Rounded	74.75	9.25	14.5	1.5
4	Swarmer	86.25	13.75	0	0
4	Vegetative	92	8	0	0
8	Rounded	52	4.5	11.5	32
8	Swarmer	NDg
8	Vegetative	96.25	3.75	0	0

^aCells of each type at a time point were categorized by live-dead staining, and the results were expressed as percentages. The data are averages of two independent experiments (n = 200 for each experiment).

^bTime after contact. After 8 h there were not enough swarmers to score.

^cThe three cell types were not necessarily equally abundant.

^dLive cells were stained only with Syto 9, and dead cells were stained only with propidium iodide.

^eCells were strongly stained with both Syto 9 and propidium iodide.

^fCells were visible by phase-contrast microscopy but not fluorescently stained.

^gND, not determined.