FIG. 1.

Physical map of the cit locus of C. glutamicum and DNA sequence of the citA-citH intergenic region (A) and physical map of the tct locus and DNA sequence of the tctC promoter region (B). Transcriptional start sites as determined by primer extension analyses are indicated by asterisks. For citH, three transcriptional start sites were identified. The one resulting in the longest transcript and having the most intense signal was set as +1. The derived −10 and −35 regions of the promoters are indicated by double lines. The proposed start codons of citH, citA, and tctC are indicated in bold, and the putative ribosome binding sites (RBS) are underlined. The proposed N-terminal amino acid residues of CitH, CitA, and TctC are also shown. Downstream of citH and tctA, Rho-independent transcriptional terminator structures are indicated by stem-loop structures.