FIG. 7.
Expression analyses and determination of the transcriptional start sites of the C. glutamicum citH gene (A) and tctCBA operon (B) by primer extension analyses with the oligonucleotides citH-PE-1 and tct-PE-3. Results for genes from the following strains cultivated under the indicated conditions are shown: lanes 1, wild type grown on glucose; lanes 2, wild type grown on citrate; lanes 3, wild type grown on citrate and glucose; lanes 4, ΔcitAB mutant grown on glucose; and lanes 5, ΔcitAB mutant grown on citrate and glucose. RNAs from the strains were isolated after cultivation in CGXII minimal medium with 50 mM MgCl2 and a 25 mM concentration of the indicated carbon source. In all primer extension experiments for which results are shown, equivalent amounts of total RNA (20 μg) were used. The transcriptional start sites are indicated by asterisks.