TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Sequence (5′→3′)a | Restriction endonuclease |
|---|---|---|
| citAB-OE-fw-SalI | TAT AGT CGA CCT TCA CGC TCT GCT GAT AAT CGC | SalI |
| citAB-OE-rv-EcoRI | TAT AGA ATT CAT GCT GAC CGA GGT GGA CAT CC | EcoRI |
| citH-OE-fw-PstI | TAT ACT GCA GCA GAA CAC TCG GGA TCT CAA AGT TTC G | PstI |
| citH-OE-rv-EcoRI | TAT AGA ATT CGG GGT TTG GCG TCG AAA AGC | EcoRI |
| tct-OE-fw | TAT ATC TAG ACC CAA GCA CTT AGG CAT CAA ACA TTC | XbaI |
| tct-OE-rv | TAT ACC CGG GAT GGT CTC TAA GCG ACT TCG AGG | XmaI |
| cg-citB-for | CGC CGC CAT ATG GAT CAA ACA CTT AAA GTT TTA GTA | NdeI |
| cg-citB-rev | ATA ATA CTC GAG CTA GAG CAG TGG CTT TGA ATA | XhoI |
| citH-PE-1 | GTG GCT GAG CGT GTA GTC AGC GGC TAA TGC CG | |
| citH-PE-2 | CTT TCT GTG TTT CTC GAA ACT TTG AGA TCC CGA G | |
| citH-PE-4 | TAA TGC CGT GAC TTG TCC TGA AG | |
| tct-PE-2 | TGC GGG TTC TGC TCC TAC TTC GGC C | |
| tct-PE-3 | AAT CCC TCT CGG TCG GAC TTT TG | |
| citAB-PE-1 | AAA TAG CTA CCA CCA ACG CGA CGG TAG CCA C | |
| citAB-PE-2 | TCC ATC ATC AAA ACT GCG AAA ATT CCG GTG C | |
| Delta-citAB-1 | TAT ATC TAG AGA GCG TGT AGT CAG CGG CTA ATG | XbaI |
| Delta-citAB-2 | CCC ATC CAC TAA ACT TAA ACA CAA AAC TGC GAA AAT TCC GGT GC | |
| Delta-citAB-3 | TGT TTA AGT TTA GTG GAT GGG GGG CGA CCA GAA CAT CTA TAT TC | |
| Delta-citAB-4 | ATA TGA ATT CAC CAT GTG GTG TTG TGC GTA CC | EcoRI |
| Delta-citAB-out-fw | GAC TAC CAG GAT GCC ATC TGA G | |
| Delta-citAB-out-rv | TAT ATC CAA CCT GCA CCA AGT AC | |
| Delta-citM-1 | TAT ACT GCA GAT GAA GAT AAG CCC GAC TCC CC | XbaI |
| Delta-citM-2 | CCC ATC CAC TAA ACT TAA ACA GTA GCC CTC TTC ATC GGC GTC G | |
| Delta-citM-3 | TGT TTA AGT TTA GTG GAT GGG GAG GAT CAT GGC GAA GCC AAG | |
| Delta-citM-4 | ATA TGA ATT CAC CAT GTG GTG TTG TGC GTA CC | EcoRI |
| Delta-citM-out-fw | CGC CCG CAT ATG AAC CAA TAG C | |
| Delta-citM-out-rv | TGA ATC ACC AGT ATT CGG GTA GC | |
| Delta-tct-1 | TAT ATC TAG ACG AGC GCT ACG TGA CTT CTT TGA | XbaI |
| Delta-tct-2 | CCC ATC CAC TAA ACT TAA ACA TGC TCC TAC TTC GGC CAT TTT GGG GG | |
| Delta-tct-3 | TGT TTA AGT TTA GTG GAT GGG AAG CAC CTG ACT TCT CAG CTC GAA ACC | |
| Delta-tct-4 | TAT ACC CGG GGG TTT AGC AAG CGG GAC GCT TTC G | XmaI |
| Delta-tct-out-fw | GGG TTT GAT TTT GAG GTG ACA ACG G | |
| Delta-tct-out-rv | TTC CTA GGC TGC CCG CTT GGG CT | |
| P_citH_fw | TTT CCA GTC GGA TCC ACC AAC AG | |
| P_citH_rv | GTT TCT CGA AAC TTT GAG ATC CCG | |
| P_citH_control_fw | CGG GAT CTC AAA GTT TCG AGA AAC | |
| P_citH_control_rv | CGA AGA TGG TGG GGA CCA ACA G | |
| P_tct_fw | GAC AAC AAG GGG GAG TCC TAG C | |
| P_tct_rv | GGT TCT GCT CCT ACT TCG GCC | |
| P_cg2836_control_fw | GGT TAG CGC GCC GCC CTG TC | |
| P_cg2836_control_rv | CGA CTT CTC AAA GGC GAC TTC AC |
a
In some cases, oligonucleotides were designed to introduce recognition sites for restriction endonucleases (recognition sites are underlined, and the corresponding restriction endonucleases are listed) and complementary 21-mer sequences for generating crossover PCR products (indicated by italics) into the resulting PCR products.