TABLE 1.

Bacterial strains and plasmids

Strain or plasmid	Descriptiona	Source or reference
Strains
S. flexneri
2457T	S. flexneri serotype 2a	10
AWY3	2457T virB::Tn5; Knr	34
Plasmids
pACYC184	Cloning vector; p15A replicon Tetr/Cmr	24
pHJW7	icsP promoter region transcriptionally fused to lacZ in pACYC184 Cmr; carries 1,232 bp of wild-type sequence upstream of the icsP transcription start site	34
pHJW20	pHJW7 carrying unique XbaI site upstream of lacZ gene; carries 1,232 bp of wild-type sequence upstream of the icsP transcription start site	This work
pJS01	pHJW20 carrying 1,056 bp of wild-type sequence upstream of the icsP transcription start site	This work
pJS02	pHJW20 carrying 893 bp of wild-type sequence upstream of the icsP transcription start site	This work
pDH01	pHJW20 carrying 665 bp of wild-type sequence upstream of the icsP transcription start site	This work
pJS04	pHJW20 carrying 351 bp of wild-type sequence upstream of the icsP transcription start site	This work
pHJW34	pHJW20 carrying 254 bp of wild-type sequence upstream of the icsP transcription start site	This work
pHJW35	pHJW20 carrying 150 bp of wild-type sequence upstream of the icsP transcription start site	This work
pHJW36	pHJW20 carrying 92 bp of wild-type sequence upstream of the icsP transcription start site	This work
pMIC01	pHJW20 with 33 bp deleted between the SalI and PstI sites in the multiple cloning site of pACYC184	This work
pMIC02	pHJW20 carrying 1,437 bp of wild-type sequence upstream of the icsP transcription start site	This work
pMIC13	pHJW20 carrying a 7-bp mutation in box 2 of the putative VirB binding site	This work
pMIC17	pHJW20 carrying a 7-bp mutation in box 1 of the putative VirB binding site	This work
pMIC18	pHJW20 carrying a 14-bp mutation in both box 1 and box 2 of the putative VirB binding site	This work
pMIC21	pHJW20 lacking all icsP promoter sequences	This work

^aAmp^r, ampicillin resistance; Tet^r, tetracycline resistance; Cm^r, chloramphenicol resistance; Kn^r, kanamycin resistance.