FIG. 5.

EMSA experiments demonstrating the binding of ExsA_His and CTD_His to the P_exoT and P_exsD promoters. (A and C) EMSA experiments using radiolabeled P_exoT (A) or P_exsD (C) promoter probes and a nonspecific (Non-Sp) DNA fragment. Probes were incubated in the absence (lane 1) or presence of the indicated concentrations of ExsA_His (lanes 2 to 8) or CTD_His (lanes 9 to 15) as indicated for 15 min at 25°C, followed by electrophoresis and phosphorimaging. Shift products 1 and 2 are indicated. (B and D) Quantitation of shift products 1 and 2 from Fig. 5A (P_exoT) and Fig. 5C (P_exsD) was performed as described in legend to Fig. 4.