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. 2009 Apr 17;191(12):4041–4043. doi: 10.1128/JB.00066-09

FIG. 2.

FIG. 2.

Effect of 2AP treatment on protein-protein interactions in the bacterial two-hybrid assay. Results in units of β-galactosidase ± standard errors of the means (n = 9) are shown for BTH101(F galE15 ga1K16 rpsL1 hsdR2 mcrA1 mcrB1 cyaA-99) cells treated with 2AP as described previously (5, 19). Cells were cotransformed with pT18 and pT25 vectors (light gray bars), pT18-mutS and pT25-mutL (white bars), pT18-vsr and pT25-mutL (gray bars), pT18-mutH and pT25-mutL (black bars), or pT18-mutL and pT25-mutL (mottled bars). (NB: The dose-response curve for the pT18-mutS pT25-mutS transformants is similar to that of the pT18-mutL pT25-mutL transformants; it has been omitted for graphical clarity since the MutS-MutS interaction gives very high units of β-galactosidase activity [15]).