Figure 6.
Sensitivity of ER− and ER+ breast cancer cell lines to Go6976. Sensitivity to Go6976 of ER− MDA-MB435 and MDA-MB231 and ER+ MCF-7 and T47D breast cancer cells and the immortalized normal human mammary epithelial cells H16N (54) grown in rich medium was determined by measurement of the number of viable cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay after treatment with different concentrations of the drug for 7 days (43). Details of growth conditions are described in Materials and Methods. Results of the average of duplicate samples of one of the three experiments are shown here. The number of cells in drug-treated samples was expressed as percent of the untreated control, designated as 100%.