TABLE 2.
Single essential enzymes in all strains of S. aureusa
| Pathway | EC no. | Gene | Enzyme name | Published experimental evidence |
|---|---|---|---|---|
| Amino sugar metabolism | 1.1.1.158 | murB | UDP-N-acetylmuramate dehydrogenase | Salmonella enterica serovar Typhimurium |
| 2.5.1.7 | murA | UDP-N-acetylglucosamine 1-carboxyvinyltransferase | Streptococcus pneumoniae, Mycobacterium tuberculosis H37Rv, S. aureus | |
| Fatty acid biosynthesis | 1.1.1.100 | fabG | 3-Oxoacyl-[acyl carrier protein] reductase | |
| 2.3.1.39 | fabD | [Acyl carrier protein] S-malonyltransferase | ||
| 2.3.1.41 | fabB/fabH | Beta-ketoacyl-acyl-carrier-protein synthase I | ||
| 3.1.2.14 | Oleoyl-[acyl carrier protein] hydrolase | |||
| 4.2.1.58 | fabZ | Crotonoyl-[acyl carrier protein] hydratase | ||
| 4.2.1.60 | fabA | 3-Hydroxydecanoyl-[acyl carrier protein] dehydratase | E. coli | |
| 4.2.1.61 | 3-Hydroxypalmitoyl-[acyl carrier protein] dehydratase | |||
| 6.4.1.2 | accA/accD | Acetyl-CoA carboxylase | B. subtilis, Haemophilus influenzae, S. pneumoniae, S. aureus | |
| Histidine metabolism | 1.1.1.23 | hisD | Histidinol dehydrogenase | M. tuberculosis H37Rv |
| 2.4.2.17 | hisG | ATP phosphoribosyltransferase | M. tuberculosis H37Rv, H. influenzae | |
| 3.1.3.15 | hisB | Histidinol phosphatase | ||
| 3.5.4.19 | hisI | Phosphoribosyl-AMP cyclohydrolase | M. tuberculosis H37Rv | |
| 3.6.1.31 | hisI | Phosphoribosyl-ATP diphosphatase | ||
| 4.2.1.19 | hisB | Imidazoleglycerol-phosphate dehydratase | M. tuberculosis H37Rv, H. influenzae | |
| 5.3.1.16 | hisA | 1-(5-Phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase | ||
| 2.6.1.9 | hisC | Histidinol-phosphate transaminase | ||
| Amino acid (phenylalanine, tyrosine and tryptophan) biosynthesis | 1.1.1.25 | aroE | Shikimate dehydrogenase | |
| 2.4.2.18 | trpD | Anthranilate phosphoribosyltransferase | M. tuberculosis H37Rv | |
| 2.5.1.19 | aroA | 3-Phosphoshikimate 1-carboxyvinyltransferase | ||
| 2.5.1.54 | aroP/aroH | 3-Deoxy-7-phosphoheptulonate synthase | ||
| 2.7.1.71 | aroL/aroK | Shikimate kinase | M. tuberculosis H37Rv | |
| 4.1.1.48 | trpC | Indole-3-glycerol-phosphate synthase | M. tuberculosis H37Rv | |
| 4.1.3.27 | trpE | Anthranilate synthase | M. tuberculosis H37Rv, H. influenzae | |
| 4.2.1.10 | aroQ | 3-Dehydroquinate dehydratase | M. tuberculosis H37Rv | |
| 4.2.1.20 | trpA/trpB | Tryptophan synthase | M. tuberculosis H37Rv, H. influenzae | |
| 4.2.1.51 | pheC | Prephenate dehydratase | H. influenzae | |
| 4.2.3.4 | aroB | 3-Dehydroquinate synthase | M. tuberculosis H37Rv, H. influenzae | |
| 4.2.3.5 | aroC | Chorismate synthase | M. tuberculosis H37Rv, H. influenzae, Helicobacter pylori | |
| 5.3.1.24 | trpF | Phosphoribosylanthranilate isomerase | ||
| 5.4.99.5 | aroQ | Chorismate mutase | H. influenzae | |
| Porphyrin metabolism | 2.1.1.107 | cysG | C-Methyltransferase, uroporphyrinogen-III | |
| 2.5.1.61 | hemC | Hydroxymethylbilane synthase | S. aureus | |
| 4.2.1.24 | hemB | Porphobilinogen synthase | ||
| 4.2.1.75 | hemD | Uroporphyrinogen-III synthase | ||
| 4.99.1.4 | cysG | Sirohydrochlorin ferrochelatase | ||
| Methionine metabolism | 2.5.1.6 | metK | Methionine adenosyltransferase | S. aureus |
| 3.2.2.9 | Adenosylhomocysteine nucleosidase | |||
| 4.4.1.21 | luxS | S-Ribosylhomocysteine lyase | ||
| Pentose phoshpate pathway | 2.2.1.1 | tktA | Transketolase | B. subtilis, S. aureus, H. influenzae, M. tuberculosis H37Rv |
| Multiple | 2.7.1.69 | yadI | Protein-N(pi)-phosphohistidine-sugar phosphotransferase | S. aureus |
| Unknown | 3.5.1.- | pcnA | Pyrazinamidase | M. tuberculosis H37Rv |
| 4.1.3.- | hisF | Imidazole glycerol phosphate synthase, cyclase subunit |
a
Forty-four enzymes functions were identified to be “singly essential” in all 13 strains of S. aureus.