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. 2009 Apr 17;191(12):3965–3980. doi: 10.1128/JB.00064-09

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Copyright © 2009, American Society for Microbiology

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FIG. 4. — qPCR-based analysis of the expression of clp regulon genes following acetamide-dependent induction of the Rv2745c gene in M. tuberculosis. qPCR was used to interrogate the expression levels of clpP1, clpP2, and clpC1 following induction of the Rv2745c gene. Results are shown for M. tuberculosis(pSM12) (carrying an integrative vector with Rv2745c in a transcriptional fusion with the M. smegmatis acetamidase promoter) and M. tuberculosis(pSCW38) (carrying an integration-proficient vector with the acetamidase promoter without any gene, as a control). Values represent mean differences (n-fold) ± standard deviations of results from quadruplicate experiments. Statistically significant changes in expression are denoted by * (P < 0.05), ** (P < 0.01), or *** (P < 0.005).