FIG. 2.
Repression analysis of the wild-type parFG promoter-operator region (ABCD) and the same region bearing disruptions of one of the sites within the OF operator (XBCD, AXCD, ABXD, and ABCX) assessed by CDO activity of parF-xylE transcriptional fusions. The top panel shows CDO activities relative to the levels of the unrepressed wild-type region for each fusion in the absence (filled bars) and presence (open bars) of ParG provided in trans. The inset shows a schematic representation of the parF-xylE constructs. The bottom panel illustrates the repression by ParG of each transcriptional fusion relative to its unrepressed levels.